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| BIOL 362 | Principles of Genetics, Spring 2012, 4.0 credits | |
| Download .pdf of Syllabus | ||
| Copies of the 2011 Lecture Notes | ||
| Date | Lecture topic |
Chapter |
| 23 Jan-Mon | Introduction |
1 |
| 25 Jan-Wed | DNA: The genetic material |
2 |
| DNA Structure | ||
| Watson & Crick | ||
| Chromosome Packing | ||
| Telomeres | ||
| Bacteriophage T4 | ||
| 30 Jan-Mon | DNA replication |
3 |
| DNA Replication - Video 1 | ||
| 01 Feb-Wed | Gene function |
4 |
| 04 Feb-Fri: Last day for student- and faculty-initiated drops | ||
| (course does not appear on academic record) | ||
| 06 Feb-Mon | Gene expression: Transcription |
5 |
| 08 Feb-Wed | Gene expression: Translation |
6 |
| microRNA Inhibitition of Translation | ||
| 13 Feb-Mon | DNA mutation and DNA repair | 7 |
| Transposable elements | 7 |
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| 15 Feb-Wed | Genomics: The mapping and sequencing of genomes | 8 |
| Dye Terminator DNA Sequencing | ||
| Pyrosequencing | ||
| 20 Feb-Mon | Exam 1 (chapters 1 to 7) | |
| Study Questions 1 | ||
| 22 Feb-Wed | Functional and comparative genomics: | 9 |
| PCR | ||
| 27 Feb-Mon | Recombinant DNA technology | 10 |
| cDNA Microarray | ||
| 29 Feb-Wed | Mendelian genetics | 11 |
| 05 Mar-Mon | Chromosomal basis of inheritance | 12 |
| 07 Mar-Wed | Extensions of Mendelian genetic principles | 13 |
| 12 Mar-Mon | Spring break | |
| 14 Mar-Wed | Spring break | |
| 19 Mar-Mon | Non-Mendelian inheritance | 13 |
| 21 Mar-Wed | Genetic mapping in eukaryotes |
14 |
| 25 Mar-Fri: Last day for student- and faculty-initiated drops | ||
| (W grade given for course) | ||
| 26 Mar-Mon | Genetics of bacteria and bacteriophages | 15 |
| 28 Mar-Wed | Variation in chromosome structure and number | 16 |
| 02 Apr-Mon | Exam 2 (chapters 7 to 14) | |
| Study Questions 2 | ||
| 04 Apr-Wed | Regulation of gene expression in bacteria | 17 |
| 09 Apr-Mon | Regulation of gene expression in eukaryotes | 18 |
| 11 Apr-Wed | Genetic analysis of development | 19 |
| 16 Apr-Mon | Genetics of cancer | 20 |
| 18 Apr-Wed | Population genetics 1 | 21 |
| 23 Apr-Mon | Population genetics 2 | 21 |
| 25 Apr-Wed | Quantitative genetics | 22 |
| 30 Apr-Mon | Molecular Evolution | 23 |
| 02 May-Wed | Final Review Session | |
| 09 May-Wed | Exam 3 (chapters 17 to 23) | |
| Study Questions 3 | ||
| 10:15 AM - 12:15 PM |
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| Location and time: | ||
| Mon & Wed 11:45 AM - 1:15 PM, Elvey Auditorium - Geophysical Institute | ||
| Textbook: | ||
| Peter J. Russel. 2010. iGenetics: A Molecular Approach. 3rd edition. Pearson Education, San Francisco, California. ISBN 0-321-56976-8/978-0-321-56976-9 |
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| Laboratory notebook: | ||
| Provide your own lab notebook and bring it to your first lab. | ||
| Web content: | ||
| Course web site (UAF): http://users.iab.uaf.edu/~kevin_mccracken/genetics/ | ||
| iGenetics web site: http://www.aw-bc.com/geneticsplace/ | ||
| Web site includes: iActivities, animations, practice quizzes, glossary, and solutions to end-of-chapter problem sets. | ||
| Grading: | ||
| Exam
1 20% Exam 2 25% Exam 3 25% Lab grade 20% Paper* 10% |
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| *One
5-page lab paper (double-spaced). This may be turned in anytime after spring break, but no later than 2 May. Topics should focus on a theme of modern 21st century genetics that is of personal or professional interest. Examples might include an overview of recent technological developments in molecular genetic technology (e.g., next-generation methods of whole genome DNA sequencing, recombinant DNA, gene discovery & isolation etc.); genetics of a particular disease or public health problem; or conservation and evolutionary genetics. These are just some possible topics. Anything is possible, but please confirm your topic with the instructor prior to writing. |
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| *Study questions will be provided online so that you have the opportunity to work through difficult concepts prior to your exams. These will not be graded, but your instructors will discuss these questions and answers during scheduled review sessions. | ||
*3% bonus added to final grade for 100% lecture attendance (attendance will be sampled randomly). |
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| *Exams in this course are purposely designed to be challenging, and it is not uncommon for raw scores to be low (e.g., means of 45-65%)—exam and final grades will be adjusted appropriately based on the overall means for each exam and the final grade distribution. | ||
| Exam make-up policy: | ||
Make-up exams are very strongly discouraged. If circumstances are dire or grievous enough that you are unable to take the exam, please contact me by phone (w: 907-474-6419, h: 907-452-2827) or e-mail (kmccrack@iab.alaska.edu) in advance of the exam. Otherwise, no make-up exam will be given and your grade will be zero for that exam. Your make-up exam may be oral! |
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| Incomplete policy: | ||
| Incomplete grades are strongly discouraged and will only be authorized under exceptional circumstances. Your performance and participation in the course will factor into this decision. |
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| Student code of conduct: | ||
| Students are subject to the UAF Student Code of Conduct. Plagiarism, cheating, and other forms of academic dishonesty will be not tolerated, and will result in immediate failure of the course (not just the assignment). Students that participate in these types of activities will be withdrawn from the course and turned over to the Dean of Student Affairs. | ||
| Other policies: | ||
| Needs of students with disabilities will be accommodated following university policies. Please talk to the instructor privately if you have questions or require assistance. The UAF Center for Health and Counseling also provides disability services. Student athletes and members of the U.S. military should coordinate their absences with the instructor in advance. | ||
| Contact information: | ||
| Kevin
G. McCracken Institute of Arctic Biology & Department of Biology and Wildlife University of Alaska Fairbanks Fairbanks, Alaska 99775 office (907) 474-6419 Rm. 242 West Ridge Research Bldg. (WRRB) email: kmccrack@iab.alaska.edu |
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| Office hours: | ||
| Monday 3:30 to 4:30 pm or by appointment. | ||
| Lab Syllabus: BIOL 362 Principles of Genetics, Spring 2012 | |||
| Lab protocols will be provided online. Please download these from the course web site each week before lab. | |||
| Week | Lab activity | ||
| 23 Jan | Introduction | ||
| Pipette techniques & lab safety | |||
| Meiosis & mitosis | |||
| 30 Jan | DNA extraction: QIAGEN protocol & Onion/Banana DNA extraction | ||
| Set up Drosophila melanogaster bottleneck experiment | |||
| 06 Feb | Polymerase chain reaction (PCR) | ||
| DNA replication, transcription, and translation | |||
| 13 Feb | Review Session for Exam #1 on 21 Feb (chapters 1 to 7) | ||
| Review Questions 1 | |||
| 20 Feb | Electrophoresis & DNA purification | ||
| Drosophila melanogaster bottleneck experiment - bottleneck 1 | |||
| 27 Feb | DNA cycle-sequencing (meet in WRRB lobby) | ||
| Tour West Ridge Research Building (WRRB) & IAB Core Facility for Nucleic Acid Research | |||
| 05 Mar | Contig construction & data analysis | ||
| 12 Mar | Spring break | ||
| Drosophila melanogaster bottleneck experiment - bottleneck 2 | |||
| (Your TA will handle the flies while you spring break - extra credit if you help your TA) | |||
| 19 Mar | Online Genbank tutorial, Protein Modeling | ||
| 26 Mar | Review Session for Exam #2 on 04 Apr (chapters 8 to 16) | ||
| Review Questions 2 | |||
| Genetic diseases Lab #1 | |||
| 02 Apr | Drosophila melanogaster bottleneck experiment - bottleneck 3 | ||
| Tour - UA Museum Facilities | |||
| 09 Apr | Cloning and gene transformation #1 | ||
| 16 Apr | Cloning and gene transformation #2 | ||
| Genetic diseases Lab #2 - presentations | |||
| 23 Apr | Drosophila melanogaster bottleneck experiment - final results | ||
| Field trip to Creamer's Field - Migratory Birds | |||
| 30 Apr | Turn in lab notebook to TA | ||
| Lab make-up policy: | |||
Lab activities flow consecutively from one week to the next; i.e., you will use your DNA extract for PCR, your purified PCR product for cycle-sequencing, and your sequence for contig construction and data analysis, etc. If you miss one step, you miss the next step. Labs should not be missed. Make-ups are at the discretion of your TA, but must be coordinated in advance and completed prior to the next lab. Missed labs will be subtracted from your lab notebook grade (20% of overall grade). |
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| Lab sections (location and time): | |||
| F01 Mon 2:15 - 5:15 PM BUNN 408 | |||
| F02 Tue 2:00 - 5:00 PM BUNN 408 | |||
| F03 Wed 2:15 - 5:15 PM BUNN 408 | |||
| TA contact information (office hours by appointment): | |||
| Andrea Price | andrea.repetto@alaska.edu | ||
| Don Larson | djlarson@alaska.edu | ||
| James Stone | james.stone@alaska.edu | ||