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| BIOL 362 | Principles of Genetics, Spring 2009, 4.0 credits | |
| Download .pdf of Syllabus | ||
| Date | Lecture topic |
Chapter |
| 26 Jan-Mon | Introduction |
1 |
| 28 Jan-Wed | DNA: The genetic material |
2 |
| DNA Structure | ||
| Watson & Crick | ||
| Chromosome Packing | ||
| Telomeres | ||
| Bacteriophage T4 | ||
| 02 Feb-Mon | DNA replication |
3 |
| DNA Replication - Video 1 | ||
| DNA Replication - Video 2 | ||
| 04 Feb-Wed | Gene function |
4 |
| RNAi | ||
| 06 Feb-Fri: Last day for student- and faculty-initiated withdrawls | ||
| (course does not appear on academic record) | ||
| 09 Feb-Mon | Gene expression: Transcription |
5 |
| 11 Feb-Wed | Gene expression: Translation |
6 |
| 16 Feb-Mon | DNA mutation and DNA repair | 7 |
| Transposable elements | 7 |
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| 18 Feb-Wed | Recombinant DNA technology | 8 |
| cDNA Microarray | ||
| 23 Feb-Mon | Exam 1 (chapters 1 to 7) | |
| Study Questions 1 | ||
| 25 Feb-Wed | Applications of recombinant DNA technology | 9 |
| PCR | ||
| Dye Terminator DNA Sequencing | ||
| Pyrosequencing | ||
| 02 Mar-Mon | Genomics | 10 |
| 04 Mar-Wed | Mendelian genetics | 11 |
| 09 Mar-Mon | Spring break | |
| 11 Mar-Wed | Spring break | |
| 16 Mar-Mon | Chromosomal basis of inheritance | 12 |
| 18 Mar-Wed | Extensions of Mendelian genetic principles | 13 |
| 23 Mar-Mon | Quantitative genetics | 14 |
| 25 Mar-Wed | Gene mapping in eukaryotes |
15, 16 |
| 27 Mar-Fri: Last day for student- and faculty-initiated withdrawls | ||
| (W grade given for course) | ||
| 30 Mar-Mon | Variation in chromosome structure and number | 17 |
| 01 Apr-Wed | Genetics of bacteria and bacteriophages | 18 |
| 06 Apr-Mon | Exam 2 (chapters 8 to 16) - Study Questions | |
| Review Questions 2 | ||
| 08 Apr-Wed | Regulation of gene expression in bacteria and bacteriophages | 19 |
| 13 Apr-Mon | Regulation of gene expression in eukaryotes, Genetic analysis of development | 20, 21 |
| 15 Apr-Wed | Genetics of cancer | 22 |
| 20 Apr-Mon | Non-Mendelian inheritance | 23 |
| 22 Apr-Wed | Population genetics 1 | 24 |
| 27 Apr-Mon | Population genetics 2 | 24 |
| 29 Apr-Wed | Molecular Evolution | 25 |
| 04 May-Mon | Final Review Session | |
| 08 May-Fri | Exam 3 (chapters 1 to 25) | |
| Study Questions 3 | ||
| 10:15 AM - 12:15 PM |
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| Location and time: | ||
| Mon & Wed 11:45 AM - 1:15 PM, Elvey Auditorium - Geophysical Institute | ||
| Textbook: | ||
| Peter J. Russel. 2006. iGenetics: A Molecular Approach. Pearson Education, San Francisco, California. ISBN 0-8053-4665-1 |
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| Laboratory notebook: | ||
| Provide your own lab notebook and bring it to your first lab. | ||
| Web content: | ||
| Course web site (UAF): http://mercury.bio.uaf.edu/~kevin_mccracken/genetics/ | ||
| iGenetics web site: http://www.geneticsplace.com | ||
| Web site includes: iActivities, animations, practice quizzes, glossary, and solutions to end-of-chapter problem sets. | ||
| Grading: | ||
| Exam
1 20% Exam 2 25% Exam 3 25% Lab grade 20% Paper* 10% |
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| *One 5-page lab paper (double-spaced). This may be turned in anytime after spring break, but no later than the last day of lab (4 May). Topics should focus on a theme of modern 21st century genetics that is of personal or professional interest. Examples might include an overview of recent technological developments in molecular genetic technology (e.g., new methods of DNA sequencing, PCR, gene discovery & isolation etc.); or genetics of a particular disease or public health problem. Please confirm your topic with the instructor prior to writing. | ||
| *Study questions will be provided online so that you have the opportunity to work through difficult concepts prior to your exams. These will not be graded, but your instructors will discuss these questions and answers during scheduled review sessions. | ||
*3% bonus added to final grade for 100% lecture attendance (attendance will be sampled randomly). |
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| *Exams in this course are purposely designed to be challenging, and it is not uncommon for raw scores to be low (e.g., means of 45-65%)—exam and final grades will be adjusted appropriately based on the overall means for each exam and the final grade distribution. | ||
| Exam make-up policy: | ||
Make-up exams are very strongly discouraged. If circumstances are dire or grievous enough that you are unable to take the exam, please contact me by phone (w: 907-474-6419, h: 907-452-2827) or e-mail (fnkgm@uaf.edu) in advance of the exam. Otherwise, no make-up exam will be given and your grade will be zero for that exam. Your make-up exam may be oral! |
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| Incomplete policy: | ||
| Incomplete grades are strongly discouraged and will only be authorized under difficult circumstances. Your performance and participation in the course will factor into this decision. |
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| Student code of conduct: | ||
| Students are subject to the UAF Student Code of Conduct. Plagiarism, cheating, and other forms of academic dishonesty will be not tolerated, and will result in immediate failure of the course (not just the assignment). Students that participate in these types of activities will be withdrawn from the course and turned over to the Dean of Student Affairs. | ||
| Other policies: | ||
| Needs of students with disabilities will be accommodated following university policies. Please talk to the instructor privately if you have questions or require assistance. The UAF Center for Health and Counseling also provides disability services. | ||
| Contact information: | ||
| Kevin
G. McCracken Institute of Arctic Biology & Department of Biology and Wildlife University of Alaska Fairbanks Fairbanks, Alaska 99775 office (907) 474-6419 Rm. 028 UA Museum email: fnkgm@uaf.edu |
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| Office hours: | ||
| Monday & Wednesday 3:30 to 4:30 pm or by appointment. | ||
| Lab Syllabus: BIOL 362 Principles of Genetics, Spring 2009 | |||
| Lab protocols will be provided online. Please download these from the course web site each week before lab. | |||
| Date | Lab activity | ||
| 26/27/28 Jan | Introduction | ||
| Pipette techniques & lab safety | |||
| Meiosis & mitosis | |||
| 02/03 Feb | DNA extraction: QIAGEN protocol & Onion/Banana DNA extraction | ||
| 09/10 Feb | Polymerase chain reaction (PCR) | ||
| DNA replication, transcription, and translation | |||
| 16/17 Feb | Review Session for Exam #1 on 23 Feb (chapters 1 to 7) | ||
| Review Questions 1 | |||
| 23/24 Feb | Electrophoresis & DNA purification | ||
| 02/03 Mar | DNA cycle-sequencing (meet in WRRB lobby) | ||
| Tour West Ridge Research Building (WRRB) & IAB Core Facility for Nucleic Acid Research | |||
| 09/10 Mar | Spring break | ||
| 16/17 Mar | Contig construction & data analysis | ||
| 23/24 Mar | Online Genbank tutorial, Protein Modeling | ||
| 30/31 Mar | Review Session for Exam #2 on 06 Apr (chapters 8 to 16) | ||
| Review Questions 2 | |||
| Genetic diseases Lab #1 | |||
| 06/07 Apr | Tour UA Museum Bird, Mammal, and Genetic Rescources Collection | ||
| 13/14 Apr | Cloning and gene transformation #1 | ||
| 20/21 Apr | Cloning and gene transformation #2 | ||
| Genetic diseases Lab #2 - presentations | |||
| 27/28 Apr | No lab final exam - Turn in notebook to TA | ||
| Lab make-up policy: | |||
Lab activities flow consecutively from one week to the next; i.e., you will use your DNA extract for PCR, your purified PCR product for cycle-sequencing, and your sequence for contig construction and data analysis, etc. If you miss one step, you miss the next step. Labs should not be missed . Make-ups are at the discretion of your TA, but must be coordinated in advance and completed prior to the next lab. Missed labs will be subtracted from your lab notebook grade (20% of overall grade). |
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| Lab sections (location and time): | |||
| F01 Mond 2:15 - 5:15 PM BUNN 408 | |||
| F02 Tue 2:00 - 5:00 PM BUNN 408 | |||
| TA contact information (office hours by appointment): | |||
| Mariana Bulgarella | ftmb3@uaf.edu | ||
| Matthew Campbell | fsmac30@uaf.edu | ||