Using eggshell membranes to characterize diet


Exploring the diet consumed by sea ducks on breeding grounds is of major interest to researchers and managers. Sea ducks migrating to arctic breeding grounds have to produce eggs shortly after arrival, and for many species it is unknown to what extent diet on breeding grounds contributes to nutrients allocated to eggs. Waterfowl leave eggshell membranes in the nest after chicks hatch, and these membranes can be collected without affecting the nest or the laying female. By analysing isotope ratios in eggshell membranes it is possible to draw inference about the diet consumed by the laying female during egg production.

We analyzed carbon and nitrogen isotope ratios in eggshell membranes of King Eiders and found that isotope ratios did not change during the course of incubation. This makes it possible to use membranes of hatched or depredated eggs to infer what diet has been used for egg production. Membranes can, however, not resolve how much endogenous nutrients were invested into eggs.

Migratory sea ducks may use a combination of endogenous and exogenous nutrients for egg production. Our data suggest that eggshell membranes reflect exogenous nutrients of the diet consumed by the female during egg synthesis. This can be useful for studies trying to quantify the origin of nutrients in eggs: the isotope ratio of eggshell membranes can be used as a clutch-specific reference point for dietary nutrients ('income endpoint') in isotopic mixing models. This approach makes it possible to take individual variability in diet choice into account when examining nutrient allocation patterns in sea ducks. [King Eider case study]

 

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