DNA Template (PCR Product)

Quantity ng/ul

100-200 bp

200-500 bp

500-1000 bp

1000-2000 bp

>2000 bp

1-3 ng

3-10 ng

5-20 ng

10-40 ng

20-50 ng

Single-stranded

25-50 ng

Double-stranded

150-300 ng

Cosmid-BAC

0.5-1.0-ug

Bacterial genomic DNA

2-3 ug

Plasmid 3 kb- 5 kb (vector + insert)

400-500 ng