Core Lab Equipment: Transgenomic WAVE DHPLC
IMPORTANT : This machine is off service contract, but if you have a LARGE project and are interested in using it please contact the Core Lab. If you are interested in potential pricing follow this link. As with all our pricing, it may be subject to change without notice.
Description:
The Transgenomic WAVE is a Denaturing High Performance Liquid Chromatorgraphy (dHPLC)
multi-platform instrument used in many nucleic acid analysis applications. Many of
these applications include Mutation Detection, Sizing, Quantitation, and
Purification. The WAVE has UV and Fluorescence detector components and a
Fragment Collector. The system uses Navigator Software to collect
and display data peaks as chromatograms. The HPLC system works by using a hydrophobic
cartridge that won’t react with DNA or RNA. TEAA is used as
an ion pairing reagent to act as a binding molecule to aid in absorption between
beads and DNA or RNA. The + Ammonium of TEAA and the – Phosphate group
in the DNA backbone bind. The Hydrophobic groups in TEAA and hydrophobic groups
in the beads are attracted to one another. The buffers used to Elute DNA or RNA off
the cartridge break the phosphorous bonds. The result is that small fragments with less bonds elute
faster than larger fragments with more phosphorous bonds, which allows fragment sizing.
Applications:
- Mutation Detection- SNPs under Partial denaturing condition so
heteroduplexes can separate from homoduplexes
- Sizing-Double stranded DNA under non-denaturing conditions. Single
stranded under fully denaturing conditions
- Quantitative Analysis- QRtPCR
- RNA Analysis-
- Oligo Quality and Purification-80ºC to fully denature and
separate by size and sequence
| Template |
Recommended DNA Quantities |
| Human Genomic DNA |
50-200 ng |
| Phage DNA |
1-10 pg |
| Plasmid DNA |
0.1-1.0 pg |
Location:
Core Lab, WRRB 207
Links:
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