How to Submit Samples for Fragment Analysis:

Fragment Analysis (microsatellite, AFLP, T-RFLP)

Submit premixed samples (PCR product , size standard and HiDi Formamide) to the Core Lab in a 96-well plate. Formamide is available at no extra cost from the Core Lab. It is covered under the sequencing fee. You should heat-denature the mixed sample at 94 or 95 for 3 min and flash cool on ice for 5 min before submitting. The Core Lab only accepts pre-mixed fragment samples for fragment analysis. Samples can sit on ice for several hours if you have to prepare them in advance, however, you should always prepare them the same day they will be run on the Instrument. PCR supplies and size standards are not provided by the Core Lab. The Core lab only accepts pre-mixed fragment samples for fragment analysis. It does not affect the price if markers are multiplexed in each well; only the number of wells matters. If you multiplex samples keep in mind that you do not want a greater total of DNA than if you were to run one marker. Also each marker will run best at different concentrations so you may want to do some optimization runs before you run your entire set of markers and samples. You must enter sample names into the computer, otherwise the samples are labeled only by well number. Ask in the Core Lab for a sample sheet template if you want to enter names on your computer ahead of time. For service and fee information follow this link.

Example recipe for preparing mixed fragment samples for submission:

9.5ul Formamide

0.5ul Size Standard (ROX 500, ROX 350, LIZ 500 etc.)

1ul (total PCR product and Water; the PCR product can be multiple markers if you want to multiplex)

11ul Total volume

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