Core Lab Equipment: Molecular Devices Analyst AD
Description:
Assay development system with four modes, fluorescence, luminescence,
absorbance, and Epi-absorbance. The instrument can be programmed to use either
a xenon-arc lamp as a continuous light source, or a xenon flash lamp. The
machine has a filter wheel that holds eight 25-mm diameter filters, has one
dichroic mirror, a 50:50 beam splitter, two sets of polarizers [S (horizontal)
or P (vertical) orientation], and a Fluorescence and luminescence PMT (Photomultiplier
tubes).
There are seven optical measurements:
- Fluorescence Intensity (F)
- High Efficiency Fluorescence Polarization (HEFP)
- Time-Resolved Fluorescence (TRF)
- Luminescence (L)
- Focused Luminescence (FLUM)
- Absorbance (A)
- Epi-Absorbance (EA)
- Software: Criterion Host and SoftMax Pro
Filters:
| Fluorophore |
Excitation Wavelength |
Emission Wavelength |
Dichroic Mirror |
| Fluorescein |
485 |
530 |
505 |
| Europium |
360 |
620 |
400 |
| Coumarin |
360 |
425 |
400 |
| Rhodamine |
530 |
580 |
565 |
| Texas Red |
580 |
630 |
505 |
| EDANS |
360 |
485 |
400 |
Dichroic Mirrors:
| Description of mirror or filter |
Wavelength |
Bandpass |
| CW 360, FWHM 35 |
360 |
35 |
| CW 425, FWHM 35 |
425 |
35 |
| CW 485, FWHM 20 |
485 |
20 |
| CW 530, FWHM 25 |
530 |
25 |
| CW 580, FWHM 10 |
580 |
10 |
| CW 620, FWHM 35 |
620 |
35 |
| CW 630, FWHM 35 |
630 |
35 |
| Dichroic 400 |
400 |
NA |
| Dichroic 505 |
505 |
NA |
| Dichroic 505/600 |
505 |
NA |
| Dichroic 565 |
565 |
NA |
| 50:50 Beam splitter |
NA |
NA |
Location:
Core Lab, WRRB 207
Links:
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