Core Lab Equipment: Amersham Typhoon 9200 Imager
Description:
The Typhoon instrument is a variable-mode imager that produces digital images of radioactive, fluorescent, or chemiluminescent samples. The Typhoon system consists of an instrument, instrument control software, and a variety of accessories. Image Quant Software includes: FragmeNT Analysis V1.2, Gene Sight V3.2, ImaGene V3.2, Image Quant V5.2, IQTools V2.2, FluorSep V2.2, and Clone Tracker V2.0.
- The Typhoon scans and processes samples in the following way:
- Red laser illuminates each one-pixel section of an exposed storage phosphor screen, the phoshphor screen emits blue light.
- When the green laser (532 nm) or the red laser (633 nm) illuminates each one-pixel section of a fluorescent sample, the fluorochrome emits light with a characteristic spectrum.
- The chemiluminescent sample emits light with a characteristic spectrum.
- Multilabel-excited mode-Creates an image from a sample labeled with up to four fluorescent dyes. The dyes can be excited by one or two of the laser wavelengths.
Note: EtBr samples are not allowed in the Typhoon, there is an Alpha Imager specifically for imaging these gels.
Accessories:
- Image Eraser-Erases storage phosphor screens for reuse
- Storage phosphor screen-General-purpose storage phosphor screen that is 20cm x 25 cm
- Exposure cassette- Two small cassettes for unmounted screens
Standard set of emission filters:
Emission filter |
Description |
555-nm band-pass filter (555 BP 20) |
Transmits light between 545 nm and 565 nm and has a transmission peak centered at 555 nm. Use this filter with fluorochromes, such as R6G and HEX. |
580-nm band pass filter (580 BP 30) |
Transmits light between 565 nm and 625 nm and has a transmission peak centered at 580 nm. Use this filter with fluorochromes, such as TAMRA and Cy3. |
610-nm band-pass filter (610 BP 30) |
Transmits light between 595 nm and 625 nm and has a transmission peak centered at 610 nm. Use this filter with fluorochromes, such as ROX, EtBr, SYPRO Red, and SYPRO Ruby. |
670-nm band pass filter (670 BP 30) |
Transmits light between 655 and 685 nm and has a transmission peak centered at 670 nm. Use this filter with fluorochromes, such as Cy5.
|
526-nm short-pass filter |
Transmits light below 526 nm. Use this filter with fluorochromes, such as fluorescein when scanning with the green laser. (Note: When using the blue laser, the 520 BP 40 might provide better results.) |
560-nm long-pass filter |
Transmits light above 560 nm. Use this filter with fluorochromes, such as TRITC. |
390-nm band-pass filter |
This filter is used for storage phosphor scanning and is not normally used for fluorescence scanning. |
The Typhoon contains three Standard beamsplitters and has a slot for one additional custom beamsplitter.
Beamsplitter |
Description |
560-nm Dichroic |
Reflects light wavelengths shorter than 560 nm and passes light longer than 560 nm. |
580-nm dichroic |
Reflects light wavelengths shorter than 580 nm and passes light longer than 580 nm |
630-nm dichroic |
Reflects light wavelengths shorter than 630 nm and passes light longer than 630 nm |
Location:
Core Lab, WRRB 207
Links:
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