Core Lab Equipment: Agilent 2100 Bioanalyzer (Happ Lab)
Description:
The Agilent 2100 Bioanalyzer is applied lab-on-a-chip technology designed to analyze RNA, DNA, proteins and cells. It can perform electrophoretic quantification of 12 samples in 30 minutes, using 1-4ul of sample, with as little as 50pg of RNA. This instrument is used to generate standardized QC values for RNA and assigns an RNA Integrity Number (RIN) to samples, allowing easy determination of RNA quality with reproducibility and comparability of results between samples and labs. This is often used before microarray or qPCR experiments. It can also be used for measuring size and concentration of DNA fragments in PCR/mPCR, multiplexed PCR and RT-PCR products as well as for protein size, purity and concentration. The digital data is easy for archiving and comparison, it also allows a variety of viewing options, including a gel-like format. Chemistries for this instrument come in RNA, DNA or Protein kits specially designed for certain size ranges or concentrations. For more information on kits the Agilent website is very useful. The Core Lab also has information available.
The instrument in the Core Lab is not equipped for analysis of cells using Flow Cytometry and would require an upgrade in order to allow this function.
User information:
The Agilent 2100 Bioanalyzer belongs to the Happ lab NOT the Core Lab. If you are interested in using it please contact either lab. The Core Lab does not run samples on the Bioanalyzer or provide chemistry kits but we are happy to help get you started.
Location:
Core Lab, WRRB 207
Links:
|
