Core Lab Equipment: AlphaImager 3400
Description:
The AlphaImager is a multi-function gel and image detection system that is equipped with a 12-bit CCD camera. It saves 16-bit files and has a host of software features that enhance image readability. Notes, labels, arrows, lines, and other drawing tools are easily added to an image. There are four auto-exposure modes, or complete manual control, ensuring customization of exposure and image capture. A movie mode allows for a series of up to 50 images to be taken, with each frame capture capable of different time intervals and exposure modes. The clarity of the image can be modified through conversion of a positive image to negative, applying digital filters, using false color maps, or adjusting the auto or manual contrast. A number of automated features are available, including:
- Molecular weight calculations
- Rf determination
- 1-D Lane and 2-D Spot densitometry
- Quantitative PCR
- Microtiter plate reading
- Object distance measuring
- Gel scoring
- Colony counting
- Lane/band detection with smiling correction
The five lighting options are detailed in the following table along with corresponding applications.
Transillumination white |
Protein gels, autorads, film, plates, flasks |
Transillumination UV |
Fluroescent gels: EtBr, SYPRO Red |
Reflective white |
Colorimetric blots and membranes |
Reflective UV |
SYBR green, TLC plates and chemifluresence |
ChromaLight |
GFP, Fluorescein, SYBR green |
In addition to the software filters the AlphaImager is currently equipped with a standard filter for use with: Colorimetric stains, film, EtBr and SYPRO Orange. There is also a dedicated photo printer attached to the instrument, or you can easily save digital image files to a jump drive. You will need a password or login to access the computer, this can be obtained through the Core Lab. The manual is also available for checkout.
User Information:
In addition to cleaning off the gel box when done using it, do not cut gels on the light box. The computer and keyboard are clean of EtBr, please do not touch with dirty gloves, but gloves should be used for your protection in the other areas. Due to limited space, please do not store your data on the computer.
Location:
North end of the WRRB 205 Shared Lab space , WRRB 213
Links:
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